Reporter

Part:BBa_K514000:Design

Designed by: Arcanum   Group: iGEM11_Sevilla   (2011-09-19)


C6-3OXO-HSL -> β-galactosidase activity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004


Design Notes

It has been very difficult to join F2620 with I142019 by the 3A method. In fact, it has been difficult for all BioBricks. Maybe it's a good method for a machine, because you don't have to purify from electrophoresis gel, but DNA concentrations suffer a great decay in the process. Use the standard method.


Source

Parts distribution of 2011.

References